Investigative Genetics


Open Access Research

Accurate, rapid and high-throughput detection of strain-specific polymorphisms in Bacillus anthracis and Yersinia pestis by next-generation sequencing

Craig A Cummings1, Christina A Bormann Chung1, Rixun Fang1, Melissa Barker1, Pius Brzoska1, Phillip C Williamson2, Jodi Beaudry3, Molly Matthews3, James Schupp3, David M Wagner3, Dawn Birdsell3, Amy J Vogler3, Manohar R Furtado1, Paul Keim3,4* and Bruce Budowle2

Author Affiliations

1 Life Technologies Corporation, Foster City, California, USA

2 University of North Texas Health Science Center, Fort Worth, Texas, USA

3 Northern Arizona University, Flagstaff, Arizona, USA

4 Translational Genomics Research Institute, Phoenix, Arizona, USA

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Investigative Genetics 2010, 1:5 doi:10.1186/2041-2223-1-5

Published: 1 September 2010

Additional files

Additional file 1:

B. anthracis SNPs. A table in Microsoft Excel format of B. anthracis SNPs identified in this study by SOLiD™ system sequencing.

Format: XLS Size: 246KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 2:

Y. pestis SNPs. A table in Microsoft Excel format of Y. pestis SNPs identified in this study by SOLiD™ system sequencing.

Format: XLS Size: 46KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Y. pestis CO92 sequence corrections. A table in PDF format of corrections made to the Y. pestis CO92 reference chromosome sequence.

Format: PDF Size: 41KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4:

TaqMan® oligonucleotide sequences. A table in PDF format of assay oligonucleotide sequences used for real-time PCR verification of putative genomic amplification regions.

Format: PDF Size: 40KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data